type esr1 cds sequence Search Results


type esr1 cds sequence  (Azenta)
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Azenta type esr1 cds sequence
Mechanism and melting temperature analysis of repressive probes. (A) Schematic of the repressive probe mechanism: qPCR amplification is coupled with a proprietary probe to selectively enrich mutant alleles by blocking wild‐type (WT) DNA amplification. (B) Mutation types covered by the switch region of the <t>ESR1</t> L536H probe. (C, D) Melting curve analysis of the L536H probe shows clear peak differentiation between mutant (61°C, orange) and WT templates (65°C, green). Mut, mutation. (E) Iterative optimization of the E380Q probe design. Version 1: 46°C versus 51°C; Version 2: 48°C versus 53°C; Version 3: 64°C versus 66°C; Version 4: 56°C versus 60°C for mutant versus WT. (F, G) Increasing the proportion of E380Q and L536H probes in the PCR system enhanced the relative amplification of the corresponding mutant sequences. F, forward primer; R, reverse primer; S, switch‐blocker.
Type Esr1 Cds Sequence, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mechanism and melting temperature analysis of repressive probes. (A) Schematic of the repressive probe mechanism: qPCR amplification is coupled with a proprietary probe to selectively enrich mutant alleles by blocking wild‐type (WT) DNA amplification. (B) Mutation types covered by the switch region of the ESR1 L536H probe. (C, D) Melting curve analysis of the L536H probe shows clear peak differentiation between mutant (61°C, orange) and WT templates (65°C, green). Mut, mutation. (E) Iterative optimization of the E380Q probe design. Version 1: 46°C versus 51°C; Version 2: 48°C versus 53°C; Version 3: 64°C versus 66°C; Version 4: 56°C versus 60°C for mutant versus WT. (F, G) Increasing the proportion of E380Q and L536H probes in the PCR system enhanced the relative amplification of the corresponding mutant sequences. F, forward primer; R, reverse primer; S, switch‐blocker.

Journal: Cancer Innovation

Article Title: A Novel Sensitive Technique to Detect ESR1 Hotspot Mutations in Liquid Biopsy Using Switch‐Blocker–Enhanced Targeted Amplification Coupled With Pyrosequencing

doi: 10.1002/cai2.70054

Figure Lengend Snippet: Mechanism and melting temperature analysis of repressive probes. (A) Schematic of the repressive probe mechanism: qPCR amplification is coupled with a proprietary probe to selectively enrich mutant alleles by blocking wild‐type (WT) DNA amplification. (B) Mutation types covered by the switch region of the ESR1 L536H probe. (C, D) Melting curve analysis of the L536H probe shows clear peak differentiation between mutant (61°C, orange) and WT templates (65°C, green). Mut, mutation. (E) Iterative optimization of the E380Q probe design. Version 1: 46°C versus 51°C; Version 2: 48°C versus 53°C; Version 3: 64°C versus 66°C; Version 4: 56°C versus 60°C for mutant versus WT. (F, G) Increasing the proportion of E380Q and L536H probes in the PCR system enhanced the relative amplification of the corresponding mutant sequences. F, forward primer; R, reverse primer; S, switch‐blocker.

Article Snippet: The wild‐type ESR1 CDS sequence and the 1607T>A mutant sequence were synthesized by GENEWIZ (Suzhou, China).

Techniques: Amplification, Mutagenesis, Blocking Assay, DNA Amplification

The repressive probe targeting ESR1 CDS 1607 shows strong amplification capability for low‐abundance mutations. (A) Amplification performance of the repressive probe in samples with high mutant allele frequency. (B) Amplification performance of the repressive probe in detecting low‐level, rare mutations.

Journal: Cancer Innovation

Article Title: A Novel Sensitive Technique to Detect ESR1 Hotspot Mutations in Liquid Biopsy Using Switch‐Blocker–Enhanced Targeted Amplification Coupled With Pyrosequencing

doi: 10.1002/cai2.70054

Figure Lengend Snippet: The repressive probe targeting ESR1 CDS 1607 shows strong amplification capability for low‐abundance mutations. (A) Amplification performance of the repressive probe in samples with high mutant allele frequency. (B) Amplification performance of the repressive probe in detecting low‐level, rare mutations.

Article Snippet: The wild‐type ESR1 CDS sequence and the 1607T>A mutant sequence were synthesized by GENEWIZ (Suzhou, China).

Techniques: Amplification, Mutagenesis